Ho'āʻo Antibody HIV / HCV (Trilines)

ʻO nā ala maʻamau no ka ʻike ʻana i ka antibody AIDS:
1. ʻIke ʻana i ka maʻi pathogen
ʻO ka ʻike ʻana i ka pathogen e pili ana i ka ʻike pololei ʻana i nā maʻi maʻi a i ʻole nā ​​​​genes viral mai nā hoʻohālike hoʻokipa ma o ka hoʻokaʻawale ʻana a me ka moʻomeheu, electron microscopic morphology observation, virus antigen detection and gene determination.He paʻakikī nā ala mua ʻelua a koi i nā mea hana kūikawā a me nā ʻenehana loea.No laila, hiki ke hoʻohana wale ʻia ka ʻike antigen a me RT-PCR (reverse transcription PCR) no ka maʻi maʻi.
2. ʻIke ʻana i ke kino
ʻO ka antibody HIV i loko o ka serum kahi hōʻike pololei ʻole o ka maʻi HIV.E like me kāna kumu nui o ka noi, hiki ke hoʻokaʻawale ʻia nā ʻano hana ʻike antibody HIV i ka hoʻāʻo screening a me ka hōʻoia hōʻoia.
3. Reagent hōʻoia
ʻO ka Western blot (WB) ke ala maʻamau i hoʻohana ʻia e hōʻoia i ka serum maikaʻi o ka hoʻāʻo screening.Ma muli o ka lōʻihi o kona manawa puka makani, ʻino ka naʻau a me ke kumukūʻai kiʻekiʻe, kūpono kēia ʻano no ka hōʻoia hōʻoia.Me ka hoʻomaikaʻi ʻana o ka naʻau o ke kolu a me ka ʻehā o ka hanauna HIV diagnostic reagents, ua lilo ʻo WB i mea hiki ʻole ke hoʻokō i nā koi no kona hoʻohana ʻana ma ke ʻano he hōʻoia hōʻoia.
ʻO kekahi ʻano o ka screening confirmation reagent i ʻae ʻia e FDA he immunofluorescence assay (IFA).ʻOi aku ka liʻiliʻi o ka IFA ma mua o WB, a maʻalahi ka hana.Hiki ke hoʻopau i ke kaʻina holoʻokoʻa i loko o 1-1.5 mau hola.ʻO ka hemahema nui o kēia ʻano, ʻo ia ke koi ʻana i nā mea ʻike fluorescence pipiʻi a me nā ʻoihana loea e nānā i nā hopena loiloi, a ʻaʻole hiki ke mālama ʻia nā hopena hoʻokolohua no ka manawa lōʻihi.I kēia manawa, ʻōlelo ʻo FDA e lanakila nā hopena maikaʻi ʻole a IFA i ka wā e hoʻopuka ai i nā hopena hope loa i nā mea hāʻawi ʻaʻole hiki ke hoʻoholo ʻia ka WB, akā ʻaʻole i manaʻo ʻia ʻo ia ka maʻamau no ka hōʻoia koko.
4. Ho'āʻo hōʻike
Hoʻohana nui ʻia ka hoʻāʻo ʻana e nānā i nā mea hāʻawi koko, no laila pono ia i ka hana maʻalahi, ke kumu kūʻai haʻahaʻa, ka ʻike a me ka kikoʻī.I kēia manawa, ʻo ELISA ke ʻano nui o ka nānā ʻana i ka honua, a aia kekahi mau ʻāpana agglutination reagents a me nā reagents ELISA wikiwiki.
Loaʻa iā ELISA ka naʻau a me ka kikoʻī, a maʻalahi ka hana.Hiki ke hoʻohana wale ʻia inā hoʻolako ʻia ke keʻena me kahi microplate heluhelu a me kahi mea holoi pā.He kūpono loa ia no ka nānā ʻana i ka nui ma ka hale hana.
ʻO ka hoʻāʻo ʻana i ka ʻāpana agglutination kekahi ala maʻalahi, maʻalahi a haʻahaʻa ke kumu kūʻai.Hiki ke hoʻoholoʻia nā hopena o kēiaʻano e nā maka'ōlohelohe, a he kiʻekiʻe loa kaʻike.He kūpono loa ia no nā ʻāina ulu a i ʻole ka nui o nā mea hāʻawi koko.ʻO ka hemahema ʻo ia ka pono e hoʻohana ʻia i nā laʻana hou, a ʻilihune ka kikoʻī.
Hepatitis C virus antibody clinical:
1) ʻO 80-90% o nā maʻi e loaʻa ana i ka maʻi hepa ma hope o ka hoʻololi ʻana i ke koko he hepatitis C, ʻo ka hapa nui o lākou he maikaʻi.
2) I nā poʻe maʻi me ka maʻi hepa B, ʻoi aku ka poʻe e hoʻohana pinepine i nā huahana koko (plasma, ke koko holoʻokoʻa) hiki ke hoʻoulu i ka maʻi maʻi hepatitis C, e lilo ai ka maʻi maʻi maʻi, cirrhosis ate a i ʻole maʻi ʻaʻai.No laila, pono e ʻike ʻia ka HCV Ab i nā poʻe maʻi me ka maʻi hepa B a i ʻole nā ​​​​maʻi me ka maʻi hepatitis mau.