Leptospira IgG/IgM Rapid Test Kit (Colloidal Gold)

Loaʻa ka Leptospirosis ma ka honua holoʻokoʻa a he pilikia maʻamau ka maʻi olakino maʻamau no ke kanaka a me nā holoholona, ​​ʻoi aku hoʻi ma nā wahi me kahi ea wela a me ka ea.ʻO nā waihona wai kūlohelohe no ka maʻi leptospirosis he ʻiole a me nā ʻano ʻano nui o nā mea momona.ʻO ka maʻi kanaka ke kumu e L. interrogans, ka lālā pathogenic o ka genus o Leptospira.Hoʻolaha ʻia ka maʻi ma o ka mimi mai ka holoholona hoʻokipa.

Ma hope o ka maʻi, aia nā leptospires i loko o ke koko a hoʻomaʻemaʻe ʻia ma hope o 4 a 7 mau lā ma hope o ka hana ʻana o ka anti-L.interrogans antibodies, ka mea mua o ka papa IgM.ʻO ka moʻomeheu o ke koko, mimi a me ka wai cerebrospinal kahi ala kūpono e hōʻoia ai i ka maʻi ma ka 1st a 2nd pule ma hope o ka hoʻolaha ʻana.ʻIke serological o antiL.ʻO nā antibodies interrogans kekahi ʻano hana diagnostic maʻamau.Loaʻa nā hoʻokolohua ma lalo o kēia māhele: 1) ʻO ka microscopic agglutination test (MAT);2) ELISA;3) Nā ho'āʻo antibody fluorescent indirect (IFATs).Eia nō naʻe, ʻo nā ʻano hana āpau i ʻōlelo ʻia ma luna nei e koi i kahi hale paʻakikī a me nā ʻenehana hoʻomaʻamaʻa maikaʻi.

ʻO ka Leptospira IgG/IgM kahi hōʻike serological maʻalahi e hoʻohana ana i nā antigens mai L. interrogans a ʻike i nā antibodies IgG a me IgM i kēia mau microorganism i ka manawa like.Hiki ke hana ʻia ka hoʻāʻo e nā limahana i hoʻomaʻamaʻa ʻole ʻia a liʻiliʻi paha, me ka ʻole o nā mea hana hoʻokolohua paʻakikī a loaʻa ka hopena i loko o 15 mau minuke.

ʻO ka Leptospira IgG/IgM Rapid Test Kit he chromatographic kahe ʻaoʻao

immunoassay.Aia i loko o ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa nā antigens L. interrogans recombinant i hui pū ʻia me ke gula colloid (Conjugates Leptospira) a me nā conjugates IgG-gula rabbit, 2) kahi ʻāpana membrane nitrocellulose i loaʻa i ʻelua mau pahu hōʻike (M a me G band) a me kahi hui mana (C band).Hoʻopili muaʻia ka M band me ka monoclonal anti-human IgM no kaʻikeʻana i ka anti-L.. interrogans IgM, ua uhi muaʻia ka band G me nā reagents no kaʻikeʻana i ka anti-L.interrogans IgG, a ua uhi mua ʻia ka hui C me ke kao anti rabbit IgG.

Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette, neʻe ka mea hōʻike ma ka hana capillary ma waena o ka cassette.IgM anti-L.. interrogans inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Leptospira.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human pre-coated, e hana ana i kahi pahu M kala burgundy, e hōʻike ana i kahi hopena hoʻokolohua L.. interrogans IgM.IgG anti-L.. interrogans inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Leptospira.A laila hopu ʻia ka immunocomplex e nā reagents i uhi mua ʻia ma ka membrane, e hana ana i kahi hui G ʻulaʻula burgundy, e hōʻike ana i kahi L.. interrogans IgG hopena hoʻokolohua maikaʻi.

ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (M a me G) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea hana ʻē aʻe.