TOXO IgG/IgM Ho'āʻo wikiwiki

ʻano nānā
ʻEkolu mau ala diagnostic nui no ka toxoplasmosis: ka maʻi pathogenic, ka maʻi immunological a me ka maʻi molecular.ʻO ka hoʻokolohua pathogenic ka nui o ka maʻi histological, inoculation holoholona a me ka noho kaʻawale, a me ka moʻomeheu cell.ʻO nā ʻano hana diagnostic serological maʻamau e pili ana i ka hoʻāʻo dye, indirect hemagglutination test, indirect immunofluorescence antibody test and enzyme linked immunosorbent assay.Hoʻopili ʻia ka ʻike molecular i ka ʻenehana PCR a me ka ʻenehana hybridization nucleic acid.
ʻO ka hoʻokolohua kino hāpai ʻana o nā makuahine hāpai e loaʻa ana kahi hoʻokolokolo i kapa ʻia ʻo TORCH.ʻO TORCH kahi hui o ka leka mua o ka inoa Pelekane o kekahi mau pathogens.ʻO ka leka T ke kū nei no Toxoplasma gondii.(ʻO nā leka ʻē aʻe e pili ana i ka syphilis, rubella virus, cytomegalovirus a me herpes simplex virus.)
Nānā kumu
ʻO ka nānā ʻana i nā pathogens
1. E nānā pono i ke koko o ka mea maʻi, ka iwi iwi a i ʻole ka wai cerebrospinal, pleural a me ascites, sputum, bronchoalveolar lavage fluid, aqueous humor, amniotic fluid, etc. ʻāpana, no ka Reich a i ʻole Ji staining microscopic examination hiki ke loaʻa nā trophozoites a i ʻole cysts, akā ʻaʻole kiʻekiʻe ka helu maikaʻi.Hiki ke hoʻohana ʻia no ka immunofluorescence pololei e ʻike i ka Toxoplasma gondii i loko o nā kiko.
2. ʻO ka hoʻomaʻamaʻa holoholona a i ʻole ka moʻomeheu ʻiʻo E lawe i ka wai o ke kino a i ʻole ka hoʻokuʻu ʻana i ka ʻiʻo e hoʻāʻo ʻia a hoʻokomo i loko o ka ʻōpū o ka ʻiole.Hiki ke loaʻa ka maʻi maʻi a loaʻa nā pathogens.Ke hewa ka hanauna mua o ka inoculation, pono ke kau makapo no ekolu manawa.A i ʻole no ka moʻomeheu ʻiʻo (ke kinikini a i ʻole nā ​​​​pūnaewele puaʻa) e hoʻokaʻawale a ʻike i ka Toxoplasma gondii.
3. DNA hybridization 'enehana Ua hoʻohana 'ia nā kānaka 'epekema i ka 32P lepili probes i loaʻa i nā kiko'ī DNA sequences o Toxoplasma gondii no ka manawa mua e alakaʻi i molecular hybridization me nā pūnaewele a me nā kiko'ī DNA i loko o nā maʻi ' peripheral koko, a hōʻike 'ia i ka pono hybridization bana a kiko.Ua kiʻekiʻe ka kikoʻī a me ka naʻau.Eia kekahi, ua hoʻokumu ʻia ka polymerase chain reaction (PCR) ma Kina e ʻike i ka maʻi, a i hoʻohālikelike ʻia me ka hoʻohālikelike ʻana i ka ʻimi noiʻi, ka hoʻopaʻa ʻana i nā holoholona a me nā ʻano hoʻokolohua immunological, hōʻike ia he kikoʻī loa, paʻakikī a wikiwiki hoʻi.
Ka hoʻokolokolo immunological
1. ʻO nā antigens i hoʻohana ʻia no ka ʻike ʻana i ka antibody ka nui o ka tachyzoite soluble antigen (cytoplasmic antigen) a me ka membrane antigen.Ua ʻike mua ʻia ka antibody o ka mea mua (ʻike ʻia e ka hoʻāʻo ʻana a me ka hoʻāʻo immunofluorescence indirect), aʻo ka mea hope i ʻike ʻia ma hope (ʻike ʻia e ka hōʻike hemagglutination indirect, etc.).I ka manawa like, hiki i nā ʻano ʻike he nui ke pāʻani i kahi hana hoʻohui a hoʻomaikaʻi i ka helu ʻike.No ka mea hiki ke noho ʻo Toxoplasma gondii i loko o nā pūnaewele kanaka no ka manawa lōʻihi, paʻakikī ke hoʻokaʻawale i ka maʻi o kēia manawa a i ʻole ka maʻi i hala ma o ka ʻike ʻana i nā antibodies.Hiki ke hoʻoholo ʻia e like me ka titer antibody a me kāna mau loli ikaika.
2. Hoʻohana ʻia ka antigen detection e ʻike i nā pathogens (tachyzoites a i ʻole cysts) i loko o nā cell host, metabolites a i ʻole nā ​​huahana lysis (circulating antigens) i loko o ka serum a me nā wai kino e nā ʻano immunological.He ala hilinaʻi no ka ʻike mua ʻana a me ka ʻike maopopo.Ua hoʻokumu ka poʻe ʻepekema ma ka home a me nā ʻāina ʻē i ka McAb ELISA a me ka sandwich ELISA ma waena o McAb a me multiantibody e ʻike i ke kaʻe ʻana o ka antigen i loko o ka serum o nā maʻi maʻi, me ka sensitivity o 0.4 μG / ml o antigen.