KA HOIKE A ME KA HOAKAKA
ʻO ka maʻi maʻi he maʻi maʻi maʻi maʻi maʻi i hoʻokumu ʻia e M. TB hominis (Koch's bacillus), i kekahi manawa na M. TB bovis.ʻO ke akemāmā ka pahuhopu mua, akā hiki ke loaʻa ka maʻi o kēlā me kēia kino.
Ua emi nui ka pilikia o ka maʻi TB i ke kenekulia 20.Eia naʻe, ʻo ka puka hou ʻana mai o nā maʻi kūʻē i ka lāʻau 1, ʻoi loa i waena o nā maʻi me AIDS2, ua hoʻāla hou i ka hoihoi i ka TB.Ua hōʻike ʻia ka maʻi o ka maʻi ma kahi o 8 miliona mau hihia i kēlā me kēia makahiki me ka make o 3 miliona i kēlā me kēia makahiki.Ua ʻoi aku ka nui o ka make ma mua o 50% ma kekahi mau ʻāina ʻApelika me ka nui o ka maʻi HIV.
ʻO ka manaʻo kānalua mua a me nā ʻike radiographic, me ka hōʻoia ʻana o ke keʻena ma hope o ka nānā ʻana i ka sputum a me ka moʻomeheu ke ʻano kuʻuna (s) i ka ʻike ʻana o ka TB5,6 ikaika.Eia nō naʻe, ʻaʻole kūpono kēia mau ʻano hana i ka naʻau a i ʻole ka manawa, ʻaʻole kūpono i nā poʻe maʻi hiki ʻole ke hana i ka sputum kūpono, smear-negative, a i ʻole i manaʻo ʻia he TB extra-pulmonary.
Hoʻokumu ʻia ka TB IgG/IgM Combo Rapid Test e hoʻopau i kēia mau pilikia.ʻIke ka hoʻāʻo iā IgM a me IgG anti-M.TB i loko o ka serum, plasm, a i ʻole ke koko holoʻokoʻa i 15 mau minuke.Hōʻike ka hopena maikaʻi IgM no kahi maʻi M.TB hou, aʻo kahi pane maikaʻi IgG e hōʻike ana i kahi maʻi maʻi mua a i ʻole maʻi mau.Ke hoʻohana nei i nā antigens kikoʻī M.TB, ʻike pū ʻo ia i ka IgM anti-M.TB i nā maʻi i hoʻopaʻa ʻia me BCG.Eia hou, hiki ke ho'āʻo
hana ʻia e nā limahana akamai ʻole a liʻiliʻi paha me ka ʻole o nā lako hana hoʻokolohua paʻakikī.
KUMU
ʻO ka TB IgG/IgM Rapid Test he lateral flow chromatographic immunoassay.Aia ka cassette ho'āʻo: 1) he papa conjugate kala burgundy i loaʻa nā antigens M.TB i hui pū ʻia me ke gula colloid (M.TB conjugates) a me nā conjugates IgG-gula rabbit, 2) kahi membrane nitrocellulose i loaʻa i ʻelua mau pahu hoʻāʻo (M a me G band. ) a me kahi pūʻulu mana (C band).Hoʻopili mua ʻia ka band M me ka IgM anti-human monoclonal no ka ʻike ʻana i ka IgM anti-M.TB, ua uhi mua ʻia ka band G me nā reagents no ka ʻike ʻana i ka IgG anti-M.TB, a ʻo ka hui C ma mua. -i uhi ʻia me ke kao anti-rabbit IgG.
Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette, neʻe ka mea hōʻike ma ka hana capillary ma waena o ka cassette.ʻO IgM anti-M.TB inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates M.TB.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human pre-coated, e hana ana i kahi pahu M kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi M.TB IgM.IgG anti-M.TB, inā aia i loko o ke kikoʻī, e hoʻopaʻa i nā conjugates M.TB.A laila hopu ʻia ka immunocomplex e nā reagents i uhi mua ʻia ma ka membrane, e hana ana i kahi band G kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi M.TB IgG.ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (M a me G) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula T.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea hana ʻē aʻe.
