wehewehe kikoʻī
ʻO Syphilis Tp kahi huakini spirochete, ʻo ia ka pathogen o ka syphilis venereal.ʻOiai ke emi nei ka nui o ka maʻi syphilis ma ʻAmelika Hui Pū ʻIa ma hope o ka puka ʻana o ka syphilis, ua piʻi ka nui o ka maʻi syphilis ma ʻEulopa mai 1986 a 1991. Ma 1992, 263 mau hihia i piʻi nui, ʻo ia hoʻi ma ka Russian Federation.Ua hōʻike ka World Health Organization i 12 miliona mau hihia hou i ka makahiki 1995. I kēia manawa, ke piʻi nei ka helu maikaʻi o ka hōʻike serological syphilis i nā poʻe i loaʻa i ka maʻi HIV.
ʻO ka ʻike wikiwiki ʻana o ka hui antibody syphilis he ʻaoʻao kahe chromatography immunoassay.
Aia i loko o ka pahu ho'āʻo: 1) kahi hui gula Tp antigen IgG conjugate e hui pū ana i kahi ʻulaʻula conjugate pad colloidal gula (Tp conjugate) me nā lākeke.
2) ʻO ke kāʻei hoʻopaʻa membrane Nitrocellulose i loaʻa i ka pahu hoʻāʻo (T) a me ke kāʻei mana (C band).Ua uhi mua ʻia ke kāʻei T me ka antigen non conjugate recombinant Tp, a ua uhi mua ʻia ke kāʻei C me ka anti rabbit IgG antibody.
Ke hāʻawi ʻia ka nui o ka hāpana i loko o ka lua hāpana, neʻe ka hāpana ma ka pahu pahu ma ka hana capillary i loko o ka pahu.Inā loaʻa ka antibody anti Tp i loko o ka hāpana, e hoʻopaʻa ia i ka conjugate Tp.A laila hopu ʻia kēia paʻakikī immune ma ka membrane e ka Tp antigen i uhi mua ʻia, e hana ana i kahi kaula T ʻulaʻula poni, e hōʻike ana i ka hopena ʻike maikaʻi o Tp antibody.ʻO ka loaʻa ʻole o ka pūna T e hōʻike ana he ʻino ka hopena.Pono ka ho'āʻo me ka mana kūloko (band C) e hōʻike i ke kao ʻulaʻula ʻulaʻula anti rabbit IgG/rabbit IgG gula conjugate o ka paʻakikī paʻakikī, me ka nānā ʻole i kāna T-band.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻohana ʻia kahi mea ʻē aʻe.
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